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Diastase activity

Concept ✔ Checked: 11.2026 · sources: 4 Updated:

Diastase activity is the activity of the enzyme diastase in honey on the Schade scale; an indicator of freshness and of no overheating.

Diastase activity shows how active diastase (amylase) is, one of the enzymes in honey that come from the saliva of worker bees. It is measured on the Schade scale. The enzyme is very fragile, so overheated, over-stored or adulterated honey has less of it or none. That makes diastase an indicator of freshness.

Directive 2001/110/EC requires a diastase value of at least 8; for honeys naturally low in enzymes, such as citrus honey, at least 3 if HMF is no more than 15 mg/kg. The limit does not apply to baker’s honey. The half-life of diastase is 4 years at 20 °C, 31 days at 40 °C and about a day at 60 °C. Directive 2024/1438 tasks the Commission with setting, by 14 June 2029, criteria that prevent enzymes from being destroyed, taking the invertase index into account.

Why this matters to you: a low diastase value does not by itself mean fraud: it shows that honey was heated or stored long, or that it is naturally poor in enzymes. That is why diastase is read together with HMF. Honey sold as honey must not be heated so that its enzymes are destroyed.

Facts

Legal basisDirective 2001/110/EC: ≥8 Schade (≥3 with HMF ≤15 mg/kg for low-enzyme honeys)
Main useFreshness indicator: heating and storage lower diastase
DurationHalf-life: 4 years at 20 °C, 31 days at 40 °C, a day at 60 °C
House rangeUS honeys (FAO): mean 20.8; range 2.1–61.2

How it connects to Honey & beekeeping

Diastase activity → kind of → Honey labels, law and quality → kind of → 🍯 Honey

Frequently asked questions

What is diastase activity?

The activity of the enzyme diastase (amylase) in honey on the Schade scale; the enzyme comes from the saliva of worker bees.

What is the EU requirement for diastase?

At least 8 on the Schade scale; at least 3 for low-enzyme honeys with HMF ≤15 mg/kg; baker’s honey is exempt.

How fast does diastase disappear in storage?

Half-life: 4 years at 20 °C, 18 months at 25 °C, 31 days at 40 °C, about a day at 60 °C.

Why read diastase together with HMF?

Initial diastase differs between honeys, whereas HMF is close to zero in fresh honey and rises with heat and time.

Is it true that low diastase means fake honey?

No. It can be overheating, long storage or a naturally low enzyme content, as in citrus honeys.

Similar

HMF in honey · Pollen analysis of honey (melissopalynology) · Pfund colour scale · Honey adulteration · Honey origin labelling · Codex standard for honey (CXS 12-1981)

Sources

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